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Name: adiponectin
Brand: R&D Systems
Rating: 94 (9 reviews)

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Figure 1. Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or <t>adiponectin.</t> Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

Figure 1. Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or <t>adiponectin.</t> Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

Adiponectin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells."

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

Journal: International journal of molecular sciences

doi: 10.3390/ijms25084147

Figure 1. Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

Figure Legend Snippet: Figure 1. Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

Techniques Used: MTT Assay, Control, Standard Deviation

Figure 2. Expression of adipokine receptors in BME-UV1 bovine mammary epithelial cells. (A,B) Expression of adiponectin receptors (ADIPOR1 and ADIPOR2); (C,D) expression of chemerin receptors (CMLKR1 and GPR1). The relative mRNA expression of the analyzed genes was normalized to the mean expression of the histone reference gene. The expression of each analyzed gene in cells cultured in control medium (Ctrl.) was given as 1. Treatment with adiponectin at concentrations of 10 ng/mL or 500 ng/mL is marked as A10 and A500, respectively. Treatment with chemerin at concentrations of 10 ng/mL or 100 ng/mL is marked as Ch10 and Ch100, respectively. Results are presented as means ± standard deviation of three independent experiments performed in duplicate. Values that differed significantly from control are marked as ** (p < 0.01) or *** (p < 0.001).

Figure Legend Snippet: Figure 2. Expression of adipokine receptors in BME-UV1 bovine mammary epithelial cells. (A,B) Expression of adiponectin receptors (ADIPOR1 and ADIPOR2); (C,D) expression of chemerin receptors (CMLKR1 and GPR1). The relative mRNA expression of the analyzed genes was normalized to the mean expression of the histone reference gene. The expression of each analyzed gene in cells cultured in control medium (Ctrl.) was given as 1. Treatment with adiponectin at concentrations of 10 ng/mL or 500 ng/mL is marked as A10 and A500, respectively. Treatment with chemerin at concentrations of 10 ng/mL or 100 ng/mL is marked as Ch10 and Ch100, respectively. Results are presented as means ± standard deviation of three independent experiments performed in duplicate. Values that differed significantly from control are marked as ** (p < 0.01) or *** (p < 0.001).

Techniques Used: Expressing, Cell Culture, Control, Standard Deviation

Figure 4. Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. (A) Representative dot-plots of Annexin V/PI double staining in untreated control cells; (B) graph showing percentage of apoptotic cells (sum of Annexin Vpos/PIneg and Annexin Vpos/Pipos cells) in control and adipokine-treated cells; (C) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; (D,E) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.

Figure Legend Snippet: Figure 4. Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. (A) Representative dot-plots of Annexin V/PI double staining in untreated control cells; (B) graph showing percentage of apoptotic cells (sum of Annexin Vpos/PIneg and Annexin Vpos/Pipos cells) in control and adipokine-treated cells; (C) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; (D,E) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.

Techniques Used: Double Staining, Control, Western Blot, Standard Deviation

Figure 5. Concentration of αS1-casein in BME-UV1 cells (A) and media (B) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Un- treated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance (p < 0.05).

Figure Legend Snippet: Figure 5. Concentration of αS1-casein in BME-UV1 cells (A) and media (B) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Un- treated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance (p < 0.05).

Techniques Used: Concentration Assay, Negative Control, Positive Control, Standard Deviation, Comparison

Figure 6. Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 µm.

Figure Legend Snippet: Figure 6. Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 µm.

Techniques Used: Immunofluorescence, Staining, Cell Culture, Control, Fluorescence

Figure 7. Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software (https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.

Figure Legend Snippet: Figure 7. Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software (https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.

Techniques Used: Cell Culture, Control, Software, Standard Deviation, Microscopy

Image Search Results

Journal: International journal of molecular sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Figure 1. Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

Article Snippet: Chemerin (cat.no: 2325-CM-025), leptin (cat.no: 498-OB-01M), adiponectin (cat.no: 5095-AC-050), and prolactin (cat. no: 682-PL-050) were purchased from R&D systems (Minneapolis, MN, USA).

Techniques: MTT Assay, Control, Standard Deviation

Journal: International journal of molecular sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Figure 2. Expression of adipokine receptors in BME-UV1 bovine mammary epithelial cells. (A,B) Expression of adiponectin receptors (ADIPOR1 and ADIPOR2); (C,D) expression of chemerin receptors (CMLKR1 and GPR1). The relative mRNA expression of the analyzed genes was normalized to the mean expression of the histone reference gene. The expression of each analyzed gene in cells cultured in control medium (Ctrl.) was given as 1. Treatment with adiponectin at concentrations of 10 ng/mL or 500 ng/mL is marked as A10 and A500, respectively. Treatment with chemerin at concentrations of 10 ng/mL or 100 ng/mL is marked as Ch10 and Ch100, respectively. Results are presented as means ± standard deviation of three independent experiments performed in duplicate. Values that differed significantly from control are marked as ** (p < 0.01) or *** (p < 0.001).

Techniques: Expressing, Cell Culture, Control, Standard Deviation

Journal: International journal of molecular sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Figure 4. Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. (A) Representative dot-plots of Annexin V/PI double staining in untreated control cells; (B) graph showing percentage of apoptotic cells (sum of Annexin Vpos/PIneg and Annexin Vpos/Pipos cells) in control and adipokine-treated cells; (C) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; (D,E) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.

Techniques: Double Staining, Control, Western Blot, Standard Deviation

Journal: International journal of molecular sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Figure 5. Concentration of αS1-casein in BME-UV1 cells (A) and media (B) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Un- treated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance (p < 0.05).

Techniques: Concentration Assay, Negative Control, Positive Control, Standard Deviation, Comparison

Journal: International journal of molecular sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Figure 6. Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 µm.

Techniques: Immunofluorescence, Staining, Cell Culture, Control, Fluorescence

Journal: International journal of molecular sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Figure 7. Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software (https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.

Techniques: Cell Culture, Control, Software, Standard Deviation, Microscopy

(A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or control vehicle. At 18 hours post-injection, the total mAT was isolated for microarray analysis. (A) Pathway analysis was performed using Enrichr and graphed based on enrichment score (Log10 (adjusted p value)). (B) Diagonal plot with red dots representing genes from the 2 most significantly enriched lipid metabolic pathways (GO: 0046460 and GO: 0006368) from (A) that were downregulated ≥2 fold (with an adjusted p value <0.05) after peptide injection compared to control. (C–D) >4 weeks post-infection with the Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or control vehicle and analyzed 4 hours post-injection. (C) Relative concentration of adiponectin in serum. (D) Relative concentration of cholesterol in serum. (E–F) Relative gene expression determined by RT-qPCR on adipocytes isolated from mAT of mice >4 weeks post infection with Yptb ΔyopM at 18 hours post-injection. (G–H) >4 weeks post-infection with Yptb ΔyopM, the mAT was cultured with YopE69–77 peptide or control vehicle and AT and culture supernatants were analyzed after 48 hours. (G) Relative gene expression was evaluated from the indicated genes. (H) Levels of free glycerol were measured in culture supernatants. Error bars in all graphs represent standard deviation. Data are representative of at least 5 experiments with 2–7 mice per group. ns not significant *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S6.

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: (A–B) >4 weeks post-infection with Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or control vehicle. At 18 hours post-injection, the total mAT was isolated for microarray analysis. (A) Pathway analysis was performed using Enrichr and graphed based on enrichment score (Log10 (adjusted p value)). (B) Diagonal plot with red dots representing genes from the 2 most significantly enriched lipid metabolic pathways (GO: 0046460 and GO: 0006368) from (A) that were downregulated ≥2 fold (with an adjusted p value <0.05) after peptide injection compared to control. (C–D) >4 weeks post-infection with the Yptb ΔyopM, mice were injected i.v. with YopE69–77 peptide or control vehicle and analyzed 4 hours post-injection. (C) Relative concentration of adiponectin in serum. (D) Relative concentration of cholesterol in serum. (E–F) Relative gene expression determined by RT-qPCR on adipocytes isolated from mAT of mice >4 weeks post infection with Yptb ΔyopM at 18 hours post-injection. (G–H) >4 weeks post-infection with Yptb ΔyopM, the mAT was cultured with YopE69–77 peptide or control vehicle and AT and culture supernatants were analyzed after 48 hours. (G) Relative gene expression was evaluated from the indicated genes. (H) Levels of free glycerol were measured in culture supernatants. Error bars in all graphs represent standard deviation. Data are representative of at least 5 experiments with 2–7 mice per group. ns not significant *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See Figure S6.

Article Snippet: N/A Toxoplasma gondii (ME-49 C1) Laboratory of Michael Grigg N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Bodipy FL C16 Life Technologies Cat# D-3821 BrdU Thermo Fisher or BD Biosciences Cat# {"type":"entrez-nucleotide","attrs":{"text":"B23151","term_id":"2508782"}} B23151 or 550891 Collagenase IV Sigma Cat# C5138-1G DNase I Sigma Cat# DN25-5G Liberase TL Roche Cat# 5401020001 Tgd_057 59–66 Genscript N/A TGME49_012300 605–619 Genscript N/A YopE 69–77 peptide Genscript N/A Critical Commercial Assays Mouse adiponectin/Acrp30 ELISA duoset R&D systems Cat# DY1119 Arcturus PicoPure RNA Isolation kit Thermo Fisher Cat# KIT0204 FITC BrdU Flow kit BD Biosciences Cat# 557891 Fixation/Permeabilization eBioscience Cat# 00-5523-00 Free Glycerol Determination kit Sigma Cat# FG0100 Infinity Cholesterol Liquid Stable Reagent Thermo Scientific Cat# TR13421 iQ SYBR Green Supermix Life Technologies Cat# 4368702 Live/Dead fixable stain Life Technologies Cat# {"type":"entrez-nucleotide","attrs":{"text":"L23105","term_id":"1185069"}} L23105 miRNeasy kit Qiagen Cat# 217084 MitoTracker Deep Red Thermo Fisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"M22426","term_id":"197107"}} M22426 Omniscript RT kit Qiagen Cat# 205111 RNeasy mini kit Qiagen Cat# 74106 Vybrant DyeCycle Violet eBioscience Cat# V35003 Deposited Data Raw RNA-Seq data This paper BioProject ID: PRJNA414132 Raw microarray data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE104955","term_id":"104955"}} GSE104955 Experimental Models: Cell Lines Experimental Models: Organisms/Strains Mouse: C57BL/6 Taconic Farms Mouse strain: B6 Mouse: Tg(CAG-DsRed*MST)1Nagy/J (DsRed reporter) Jackson Laboratory Mouse strain: Jax005441 Mouse: B6.SJL-Ptprc a /BoyAiTac (CD45.1) Taconic Farms – NIAID exchange Mouse strain: Tac8478 Mouse: C57BL/6-[Tg]CD11c( Itgax ):EYFP (CD11c–eYFP reporter) Taconic Farms – NIAID exchange Mouse strain: Tac307 B6.SJL-Cd45a(Ly5a)Nai-[KO]RAG1 (Rag KO) Taconic Farms – NIAID exchange Mouse strain: Tac165 Non-human primate: Macaca mulatta Laboratory of J.M.B.

Techniques: Infection, Injection, Isolation, Microarray, Concentration Assay, Expressing, Quantitative RT-PCR, Cell Culture, Standard Deviation

Journal: Immunity

Article Title: The white adipose tissue is a reservoir for memory T cells that promotes protective memory responses to infection

doi: 10.1016/j.immuni.2017.11.009

Figure Lengend Snippet: DATA AND SOFTWARE AVAILABILITY

Techniques: Software, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Staining, Microarray

A, left panel, Experimental timeline. Chronically cannulated mice received intracerebroventricular infusion of mouse recombinant adiponectin daily for 7 consecutive days. Right panel, Changes in body weight over 7 days of treatment; Veh, vehicle; Adipo, adiponectin; *, P < .05; **, P < .01; ***, P < .001 compared with the vehicle-treated group. B, The soma size (left), total dendritic length (middle left), number of branches (middle right), and Sholl analysis (right) of dentate granule neurons from mice treated with adiponectin or vehicle. C, upper panel, Representative images of dendritic tracings of early-born dentate granule neurons in adiponectin- or vehicle-treated mice. Lower panel, Quantification of soma size (left), total dendritic length (middle left), number of branches (middle right), and Sholl analysis (right) of early-born dentate granule neurons in adiponectin- or vehicle-treated mice. D, upper panel, Representative images of dendritic tracings of late-born dentate granule neurons in adiponectin- or vehicle-treated mice. Lower panel, Quantification of soma size (left), total dendritic length (middle left), and number of branches (middle right) of late-born dentate granule neurons showing no difference between 2 treatment groups; Sholl analysis of dendritic intersections (right) indicating a significant interaction between treatment and distance from soma (P < .001).

Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: A, left panel, Experimental timeline. Chronically cannulated mice received intracerebroventricular infusion of mouse recombinant adiponectin daily for 7 consecutive days. Right panel, Changes in body weight over 7 days of treatment; Veh, vehicle; Adipo, adiponectin; *, P < .05; **, P < .01; ***, P < .001 compared with the vehicle-treated group. B, The soma size (left), total dendritic length (middle left), number of branches (middle right), and Sholl analysis (right) of dentate granule neurons from mice treated with adiponectin or vehicle. C, upper panel, Representative images of dendritic tracings of early-born dentate granule neurons in adiponectin- or vehicle-treated mice. Lower panel, Quantification of soma size (left), total dendritic length (middle left), number of branches (middle right), and Sholl analysis (right) of early-born dentate granule neurons in adiponectin- or vehicle-treated mice. D, upper panel, Representative images of dendritic tracings of late-born dentate granule neurons in adiponectin- or vehicle-treated mice. Lower panel, Quantification of soma size (left), total dendritic length (middle left), and number of branches (middle right) of late-born dentate granule neurons showing no difference between 2 treatment groups; Sholl analysis of dendritic intersections (right) indicating a significant interaction between treatment and distance from soma (P < .001).

Article Snippet: Chemicals Recombinant mouse adiponectin (R&D Systems) was dissolved in artificial CSF (137mM NaCl, 2.7mM KCl, 0.5mM MgCl 2 · 6H 2 O, 0.9mM CaCl 2 · 2H 2 O, 1.5mM KH 2 PO 4 , and 8.1mM Na 2 HPO 4 ) at a concentration of 0.5 μg/μL.

Techniques: Recombinant

A, left panel, Total spine density. Right panel, Quantification of density of each spine type (mushroom, thin, and stubby spines) on distal dendrites of dentate granule neuron in adiponectin- or vehicle-treated mice; Veh, vehicle; Adipo, adiponectin; *, P < .05; ***, P < .001 compared with the vehicle-treated group. B, upper panel, Representative Golgi-stained images of dendritic spines on early-born dentate granule neurons from adiponectin- or vehicle-treated mice. Lower panel, Quantification of total spine density (left) and densities of each spine type (right) on distal dendrites of early-born dentate granule neurons in mice infused with adiponectin or vehicle. C, upper panel, Representative Golgi-stained images of dendritic spines on late-born dentate granule neurons from adiponectin- or vehicle-treated mice. Lower panel, Quantification of total spine density (left) and densities of each spine type (right) on distal dendrites of late-born dentate granule neurons in mice infused with adiponectin or vehicle; *, P < .05; ***, P < .001 compared with the vehicle-treated group.

Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: A, left panel, Total spine density. Right panel, Quantification of density of each spine type (mushroom, thin, and stubby spines) on distal dendrites of dentate granule neuron in adiponectin- or vehicle-treated mice; Veh, vehicle; Adipo, adiponectin; *, P < .05; ***, P < .001 compared with the vehicle-treated group. B, upper panel, Representative Golgi-stained images of dendritic spines on early-born dentate granule neurons from adiponectin- or vehicle-treated mice. Lower panel, Quantification of total spine density (left) and densities of each spine type (right) on distal dendrites of early-born dentate granule neurons in mice infused with adiponectin or vehicle. C, upper panel, Representative Golgi-stained images of dendritic spines on late-born dentate granule neurons from adiponectin- or vehicle-treated mice. Lower panel, Quantification of total spine density (left) and densities of each spine type (right) on distal dendrites of late-born dentate granule neurons in mice infused with adiponectin or vehicle; *, P < .05; ***, P < .001 compared with the vehicle-treated group.

Techniques: Staining

A, left panel, Experimental timeline. Chronically cannulated mice received ICV infusions of mouse recombinant adiponectin daily for 7 consecutive days. BrdU was infused ICV for the first 3 days. Right panel, Changes in body weight over 7 days of adiponectin treatment. Veh, vehicle; Adipo, adiponectin. B, left panel, Representative images of Ki67-positive cells. Right panel, Quantification of the number of Ki67-positive cells in the dentate gyrus of adiponectin- and vehicle-treated mice; *, P < .05 compared with the vehicle-treated group. Scale bar, 50 μm. C, left panel, Representative confocal images showing the colocalization of BrdU with NeuN or BrdU with GFAP. Right upper panel, Quantitative analysis of neuronal differentiation by calculating the percentage of BrdU-positive cells double-labeled with NeuN or GFAP in mice treated with adiponectin or vehicle. Right lower panel, Quantification of total number of new-born neurons (cells double-labeled for BrdU and NeuN). Scale bar, 10 μm; *, P < .05 compared with the vehicle-treated group.

Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: A, left panel, Experimental timeline. Chronically cannulated mice received ICV infusions of mouse recombinant adiponectin daily for 7 consecutive days. BrdU was infused ICV for the first 3 days. Right panel, Changes in body weight over 7 days of adiponectin treatment. Veh, vehicle; Adipo, adiponectin. B, left panel, Representative images of Ki67-positive cells. Right panel, Quantification of the number of Ki67-positive cells in the dentate gyrus of adiponectin- and vehicle-treated mice; *, P < .05 compared with the vehicle-treated group. Scale bar, 50 μm. C, left panel, Representative confocal images showing the colocalization of BrdU with NeuN or BrdU with GFAP. Right upper panel, Quantitative analysis of neuronal differentiation by calculating the percentage of BrdU-positive cells double-labeled with NeuN or GFAP in mice treated with adiponectin or vehicle. Right lower panel, Quantification of total number of new-born neurons (cells double-labeled for BrdU and NeuN). Scale bar, 10 μm; *, P < .05 compared with the vehicle-treated group.

Techniques: Recombinant, Labeling

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